Tissue+Culture+by+Olivia+Waitekus

Tissue Culture Tissue Culture is the technique or process of keeping tissue alive and growing in a culture medium. It is mostly used to mass produce plants, but it can also be used to grow new organs.

The process:

First plants are surface sterilized and small buds or growing tips are placed on media which have fertilizer, vitamins and growth regulators. This is solidified by agar so that the plants can stand up straight and have nutrients diffuse to the plant and also have toxins diffuse away from the plant. When the culture is clean, the plant is multiplied and every 2 to 3 weeks, the plant material is removed from the jar or test-tube to be divided and trimmed as required and put back onto fresh media. The hood uses __#|hepa filters__ which clean to point two microns. The plants are placed on __paper__ towel which have been sterilized. When the required numbers of plants have been grown, the plants are put onto Elongation media so that good micro cuttings can be harvested. http://www.dnagardens.com/Articles/tissue.htm



What you need: http://www.biotechnologyonline.gov.au/biotechnologyonline/pdf/biotech/plant_tissue_culture_in_class.pdf
 * Distilled water
 * Agar plates
 * Bleach or sterilizing equipment
 * Glass aquarium
 * Growth medium
 * Your choice of plants
 * Culture tubes
 * Sterile growth chamber
 * __#|Growth hormones__

Types of Tissue Culture Organ Culture Used to regrow organs Take cells and regrow missing or damaged organ

Plant Tissue Culture Used to mass produce plants, bacteria and algae Tissue Culture on TV

In Grey’s Anatomy, a patient Lily Price suffers from a benign growth on her windpipe that keeps recurring despite numerous surgeries to remove it. The tumor may be benign, which means non-cancerous, but the recurrence and size of the mass cause it to impinge on Lily's airway, making it extremely difficult to breathe on her own. If our surgeons at Seattle Grace-Mercy West tried to remove the whole tumor at, they would be forced to remove Lily's entire trachea. Without a trachea, Lily would die.What about a replacement? People get organs and tissue transplanted and replaced all the time, right? Well, not so simple. An artificial option for a trachea does not yet exist, and donor tracheas have not been proven to __#|work__. Therefore, Lily's surgical team made a decision, with Lily and her mother's consent, to try an experimental solution. They decided to grow Lily a new trachea, using her very own stem cells. Surgeons would take cartilage cells and fibrous tissue from Lily's twelfth rib by harvesting a piece approximately three centimeters long. They then take the cartilage up to the lab, wash it thoroughly, and break the mixture down to a cellular level, turning it into a protein liquid. Meanwhile, the doctors constructed a piece of "scaffolding" to form her new trachea. This scaffolding? Basically looks like a clump of cotton-balls, like little tangled up fibers of mesh. They take the cell-protein-liquid and drip it onto the scaffolding, let it soak, and then wrap it into a tube (similar to the shape of a windpipe!).And now, the "cooking" begins. The doctors place this whole thing into an incubator for, ideally, five to nine days. Once the appropriate amount of time has passed, surgeons then implant the newly-constructed trachea into the patient's body (in order to let the tissue mature), usually under a muscle bed because they keep blood vessels alive and well there. In Lily's case, the trachea went into the abdomen.

http://supplements-daily.com/wp-content/uploads/2011/07/341.jpg http://upload.wikimedia.org/wikipedia/commons/0/09/Plant_Tissue_Culture_Lab_-_Atlanta_Botanical_Garden.JPG

Tissue Culture explained: tissue culture video

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